Growing up bacteria is pretty easy, you take a very small amount, and inoculate a solution that permits its growth. Leave in the proper temperature, sometimes an incubator set at 37C and you have a cloudy slimy stinky broth the next day.
Spinning it down with a centrifuge drives all of the bacteria floating in the fluid to the bottom of the tube, you repeat this until you have finished the stock you grew over night. Once I was done with that I had to preform what is called a Mini Prep where you brake down the bacteria and filter out all of the things you don't want with detergents and solutions. After I was done, boom I had 600uL of .015 ng rich solution of bacterial plasmid PJE202. <==Plasmid for Bio-luminescence.
I love my life!
Keep on biohacking!